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Clinical Chemistry 20: 1458-1460, 1974;
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Clinical Chemistry, Vol 20, 1458-1460, Copyright © 1974 by the American Association for Clinical Chemistry

2-Amino-2-methyl-1-propanol as the Alkalizing Agent in an Improved Continuous-Flow Cresolphthalein Complexone Procedure for Calcium in Serum

Wells R. Moorehead 1 and Homer G. Biggs 1

1 Department of Clinical Pathology, University of Indiana Medical Center, Indianapolis, Ind. 46202.

We describe an improved cresolphthalein complexone procedure for calcium in which diethylamine is replaced by a nontoxic amino alcohol. Advantages of the procedure are increased sensitivity, excellent baseline stability, a more optimum reaction environment (pH 10.0) that eliminates blanking problems, and freedom from interference by magnesium. Values obtained by this method are not significantly different (P < 0.05) from those obtained by atomic absorption spectrophotometry.


Key Words: AutoAnalyzer • atomic absorption spectrophotometry compared

Submitted on July 3, 1974
Accepted on August 12, 1974




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