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Clinical Chemistry 20: 1528-1534, 1974;
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Clinical Chemistry, Vol 20, 1528-1534, Copyright © 1974 by the American Association for Clinical Chemistry

Creepage of Estrogens vs. Loss by Sorption on Glassware

Stanley Kushinsky 1 and Marilyn Anderson 1

1 Rees-Stealy Clinic Research Foundation, 2001 Fourth Ave., San Diego, Calif. 92101.

Creepage and decomposition by atmospheric pollutants account for many if not all of the losses that have been attributed previously by others to sorption on glassware. Significant creepage occurs only in uncovered (or incompletely closed) vessels, in the presence of salts and in the absence of proteins (such as gelatin). More creepage occurs in vessels that have been freshly cleaned by treatment with heat, cleaning solution, or organic solvents, when the volume of solution is relatively small compared with the capacity of the vessel and at higher dilutions. In addition, the rate of creepage of intact estrogens is greater than that of the decomposition products. We have found no evidence for sorption of estrogens onto glass from buffered aqueous solutions or decomposition in tightly closed containers in the absence of proteins. Under the conditions we used for silanization, creepage was reduced drastically but not eliminated totally.


Key Words: decomposition • ambient storage conditions

Submitted on August 8, 1974




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Copyright © 1974 by the American Association for Clinical Chemistry.