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Clinical Chemistry 20: 1548-1552, 1974;
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Clinical Chemistry, Vol 20, 1548-1552, Copyright © 1974 by the American Association for Clinical Chemistry

Optimizing Nephelometric Measurement of Specific Serum Proteins: Evaluation of Three Diluents

Lawrence M. Killingsworth 1, Gregory J. Buffone 1, Meena B. Sonawane 1, and Glennie C. Lunsford 1

1 Departments of Hospital Laboratories, Medicine, Pathology, and Biochemistry, University of North Carolina School of Medicine, Chapel Hill, N. C. 27514.

L. M. K., Clinical Chemistry Laboratories, N. C. Memorial Hospital, Chapel Hill, N. C. 27514.

Three diluents were studied, to determine which is the best for the automated immunochemical measurement of specific serum proteins. Nine serum proteins (orosomucoid, agr1-antitrypsin, agr2-macroglobulin, haptoglobin, transferrin, C3, IgG, IgA, and IgM) were measured in physiological saline (9 g NaCI/liter), tris(hydroxymethyl)aminomethane buffer (0.01 mol/liter; pH 7.4), and physiological saline containing polyethylene glycol ("PEG 6000," 40 g/liter). Criteria were: reaction rate, analysis rate, carryover between samples, steady-state approximation, precision, and correlation with other methods. Saline containing polyethylene giycol is the best of the three diluents for use in continuous-flow nephelometric analysis of serum proteins.

Submitted on August 17, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.