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Clinical Chemistry 20: 1553-1555, 1974;
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Clinical Chemistry, Vol 20, 1553-1555, Copyright © 1974 by the American Association for Clinical Chemistry

Simplified Colorimetry of agr-Amino Nitrogen in Plasma, Serum, or Urine

Klaus Lorentz 1 and Barbara Flatter 1

1 Medizinische Klinik der Medizinischen Hochschule Lübeck, Kronsforder Allee 71/73, Lübeck, Germany.

We describe a simple procedure for estimating amino acids, based on the formation of charge-transfer complexes with p-benzoquinone. The reaction is insensitive to ammonia, urea, purines, creatinine, and other nitrogenous compounds present in serum or urine, except amines, peptides, and proteins. The reagent is cheap, stable, and does not require prior treatment of urine before determination. Specifity, precision, and normal values of this method are reported and compared with those obtained by a colorimetric ninhydrin technique.


Key Words: glycine equivalents • normal values • p-benzoquinone reagent • liver disease

Submitted on July 8, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.