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Clinical Chemistry 20: 1568-1572, 1974;
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Clinical Chemistry, Vol 20, 1568-1572, Copyright © 1974 by the American Association for Clinical Chemistry

Parameters Affecting a Rapid Method In Which Sephadex Is Used to Determine the Percentage of Free Thyroxine in Serum

Stanley S. Levinson 1 and Sidney V. Rieder 1

1 Chemistry Laboratory, Massachusetts General Hospital, Boston, Mass. 02114.

In this method, Sephadex is used to assess the percentage of free thyroxine in undiluted sera. We demonstrate that if temperature and amount of Sephadex are kept constant, reproducibility is excellent. When Sephadex is exposed to serum, equilibration between thyroxine and thyroxine-binding components of the system is instantaneous. Because it is so rapid, it is not possible to measure the true physiological fraction of free thyroxine in serum with any free-thyroxine-binding resin, but the method does give an accurate measure of fluctuations in percentage of free thyroxine, as indicated by the high correlation with values for percentage of free thyroxine as determined by equilibrium dialysis and by the appropriate response to additions of exogenous thyroxine and dilution of the serum.


Key Words: equilibrium dialysis • normal values • diagnostic aid • thyroid disease

Submitted on July 1, 1974
Accepted on October 5, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.