Clinical Chemistry AACC Online Job Center
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 20: 1576-1577, 1974;
This Article
Right arrow Full Text (PDF)
Right arrow Submit an electronic Letter to
the Editor about this paper
Right arrow Alert me when this article is cited
Right arrow Alert me when eLetters are posted
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Murat, J. C.
Right arrow Articles by Serfaty, A.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Murat, J. C.
Right arrow Articles by Serfaty, A.

Clinical Chemistry, Vol 20, 1576-1577, Copyright © 1974 by the American Association for Clinical Chemistry

Simple Enzymatic Determination of Polysaccharide (Glycogen) Content of Animal Tissues

J. C. Murat 1 and A. Serfaty 1

1 Laboratoire d’Ecophysiologie Animale, Université Paul Sabatier, 38 rue des 36 Ponts, 31400 Toulouse, France.

For determination of (chiefly) glycogen content, tissue homogenates in citrate buffer are mixed with exo-1,4-agr-glucosidase (EC 3.2.1.3). Liberated glucose is estimated with a Beckman Glucose Analyzer. This method was checked with commercial glycogen (from oysters) and with fish (carp) tissues. Comparative measurements were made with the classic Good-Kramer-Somogyi method [J. Biol. Chem. 100, 485 (1933)]. The described method, suitable for small samples of tissue, such as those obtained by biopsy, seems to be simple and accurate, but gives values 5 to 10% higher than those given by the classic KOH-ethanol method.


Key Words: glycogen storage diseases • assay of polysaccharides containing 1,4- and 1.6-linked agr-D-glucose • assay of biopsy samples

Submitted on July 23, 1974




The following articles in journals at HighWire Press have cited this article:


Home page
 J. Nutr.Home page
S. Kirchner, S. Kaushik, and S. Panserat
Low Protein Intake Is Associated with Reduced Hepatic Gluconeogenic Enzyme Expression in Rainbow Trout (Oncorhynchus mykiss)
J. Nutr., August 1, 2003; 133(8): 2561 - 2564.
[Abstract] [Full Text] [PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the American Association for Clinical Chemistry.