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Clinical Chemistry 20: 141-147, 1974;
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Clinical Chemistry, Vol 20, 141-147, Copyright © 1974 by the American Association for Clinical Chemistry

Quantitative Determination of Medazepam, Diazepam, and Nitrazepam in Whole Blood by Flame-Ionization Gas—liquid Chromatography

Mavis S. Greaves 1

1 Rheumatism Research Unit, Nether Edge Hospital, Sheffield S11 9EL, England.

Accurate methods are described for the qualitative and quantitative determination of medazepam, diazepam, and nitrazepam in 5 ml of whole blood. Medazepam and diazepam are analyzed intact and nitrazepam is chromatographed as its trimethylsilyl derivative by flame-ionization gas-chromatography on "1% OV-17." A supplementary column of "2% OV-1" is used to separate nitrazepam TMS from diazepam when both are present in the same extract. Essential data given include the percentage recovery of medazepam, and the flame-ionization detector responses of diazepam and silylated nitrazepam relative to medazepam, calculated after extraction from blood and gas-chromatographic analysis. Chromatograms are illustrated of extracts of blood taken from patients on medazepam and diazepam therapy and from a patient who had taken an overdose of nitrazepam.


Key Words: toxicology • tranquilizers • monitoring therapy

Submitted on March 12, 1973
Accepted on October 23, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.