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Clinical Chemistry 20: 236-242, 1974;
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Clinical Chemistry, Vol 20, 236-242, Copyright © 1974 by the American Association for Clinical Chemistry

Gas-Chromatographic Simultaneous Analysis for Glutethimide and an Active Hydroxylated Metabolite in Tissues, Plasma, and Urine

Alfred R. Hansen 1 and L. J. Fischer 1

1 The Toxicology Center, Department of Pharmacology, University of Iowa, Iowa City, Iowa 52242.

We report a gas-chromatographic method for the simultaneous quantitative analysis of both glutethimide and 4-hydroxy-2-ethyl-2-phenylglutarimide (4- HG), an active metabolite of glutethimide, in tissues, plasma, and urine. The method, applied to postmortem tissues from cases of fatal glutethimide overdose and to plasma samples obtained from a patient in coma from acute glutethimide intoxication, proved to be accurate, sensitive, and specific. Results of the analysis confirm that 4-HG accumulates in plasma and tissues during glutethimide intoxication. Because 4-HG has been shown previously to be at least as potent as glutethimide itself, its accumulation may play an important role in the acute toxicity of glutethimide.

Submitted on November 23, 1973
Accepted on November 27, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.