Clinical Chemistry
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Clinical Chemistry 20: 249-254, 1974;
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Clinical Chemistry, Vol 20, 249-254, Copyright © 1974 by the American Association for Clinical Chemistry

Qualitative and Quantitative Determination of Methaqualone in Serum by Gas Chromatography

M. A. Evenson 1 and G. L. Lensmeyer 1

1 Department of Medicine and the Clinical Laboratories, The University of Wisconsin-Madison, 1300 University Ave., Madison, Wis. 53706.

A rapid, simple, accurate, and precise isothermal gas-chromatographic method is introduced for determination of methaqualone (2-methyl-3-o-tolyl-4(3H)-quinazolinone) in serum. A single extraction of 2 ml of serum, without derivative formation, will give adequate sensitivity for quantitation of therapeutic serum concentrations of the drug within 15 min. The method is free of interferences from biological substances, as well as from commonly used drugs. A non-drug internal standard compensates for variables in extraction, injection, and instrumental changes during analysis. The coefficient of variation, day-to-day, is 5.6%. Mean recovery of added methaqualone was 80%. To compensate for the nonquantitative yield and ensure accurate results, we prepared all analytical methaqualone standards in serum.

Submitted on December 2, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.