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Clinical Chemistry 20: 272-274, 1974;
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Clinical Chemistry, Vol 20, 272-274, Copyright © 1974 by the American Association for Clinical Chemistry

Use of Fluorescamine ("Fluram") to Detect Amphetamine in Urine by Thin-Layer Chromatography

Bernard Klein 1, Joan E. Sheehan 1, and E. Grunberg 1

1 Department of Diagnostic Research, Hoffmann-La Roche Inc., Nutley, N. J. 07110.

A "Fluram" (fluorescamine; 4-phenylspiro[furan-2(3H)-1'-phthalan]-3,3'-dione) spray reagent will detect as little as 250 ng of amphetamine in an extract of amphetamine-containing urine, after it has been separated by thin-layer chromatography. The fluorescence is stable for about 20 h and can be renewed by respraying. Fluram spray does not interfere with reagent sprays used to detect other drugs of abuse. Amphetamine on the thin-layer plate can be measured by extracting the fluorescent area of silica gel and measuring the fluorescence in a fluorimeter. Recovery of 1.0 µg and 2.0 µg of amphetamine averaged 69% and 67%, respectively.


Key Words: thin-layer chromatography on silica gel • toxicology • drug abuse

Submitted on October 22, 1973
Accepted on December 5, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.