Clinical Chemistry
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Clinical Chemistry 20: 328-331, 1974;
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Clinical Chemistry, Vol 20, 328-331, Copyright © 1974 by the American Association for Clinical Chemistry

Automated Measurement of Protease-Inhibitor Capacity of Serum, by Use of Chymotrypsin and Casein

Julius Wenger 1 and Marian Sundy 1

1 Veterans Administration Hospital, and the Department of Medicine, Emory University School of Medicine, Decatur, Ga. 30033.

Serum protease inhibitor activity was assessed from the ability of the serum to inhibit casein hydrolysis by agr-chymotrypsin (EC 3.4.4.5). Unmodified AutoAnalyzer equipment was used in conjunction with a fluorimetric method for dialyzable tyrosyl peptides. Serum, diluted with tris(hydroxymethyl) aminomethane buffer, was added to a chymotrypsin solution to inhibit its activity. For 72 sera, the automated method was compared with a manual determination of trypsin inhibitor capacity. Results by the two methods correlate well, supporting previous evidence that trypsin and chymotrypsin-inhibitor capacities of serum are related. The present automated method has several advantages that make it useful for clinical studies.


Key Words: inhibitors of trypsin and chymotrypsin in serum • AutoAnalyzer • fluorimetry • emphysema • pancreatitis • manual method compared • diagnostic aid

Submitted on July 30, 1973
Accepted on December 5, 1973






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Copyright © 1974 by the American Association for Clinical Chemistry.