Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 20: 348-352, 1974;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via HighWire
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Parekh, C. K.
Right arrow Articles by Murphy, B. F.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Parekh, C. K.
Right arrow Articles by Murphy, B. F.

Clinical Chemistry, Vol 20, 348-352, Copyright © 1974 by the American Association for Clinical Chemistry

Automated Simultaneous Determination of p-Aminohippurate and Creatinine in Plasma or Urine

C. K. Parekh 1, J. Kirpan 1, A. Peterson 1, G. L. Hassert 1, and B. F. Murphy 1

1 Department of Toxicology, The Squibb Institute for Medical Research, New Brunswick, N. J. 08903.

Methods have been developed for using the Beckman "Discrete Sample Analyzer" (DSA-560), for automated simultaneous determinations of p-aminohippurate (PAH) and creatinine in the same 50- or 10-µl samples of plasma or urine, respectively, at the rate of 80 samples per hour. In determinations of PAH, a single reagent, p-dimethylaminocinnamaldehyde in 0.1 mol/liter HCl, was added to a protein-free filtrate from plasma or urine. The intensity of the color, measured at 550 nm, obeyed Beer’s law for PAH concentrations from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ±5% of the results obtained by the automated method. Creatinine was simultaneously determined by adapting the method of Taussky to the DSA-560 instrument; the intensity of the color, measured at 510 nm, obeyed Beer's law for creatinine concentrations ranging from 0.005 to 5.0 g/liter. Data obtained for the same plasma and urine samples by the manual method, were, in general, within ± 5% of the results obtained by the automated method.


Key Words: renal function • effective renal plasma flow • glomerular filtration rate • protein-free filtrate • Beckman DSA

Submitted on April 25, 1973
Accepted on December 14, 1973




The following articles in journals at HighWire Press have cited this article:


Home page
 Am. J. Physiol. Renal Physiol.Home page
R. Agarwal
Rapid microplate method for PAH estimation
Am J Physiol Renal Physiol, August 1, 2002; 283(2): F236 - F241.
[Abstract] [Full Text] [PDF]

Home page
 Toxicol PatholHome page
Y. H. Yoon and E. Santamarina
Hypoplastic Renal Corpuscles in the Dog
Toxicol Pathol, February 1, 1979; 7(2): 1 - 5.
[Abstract] [PDF]

Home page
 Toxicol PatholHome page
P. L. Sibley, V. M. Traina, and C. K. Parekh
Normal Range and Variability of Hematologic and Blood-Chemical Parameters from Beagle Dogs and Rhesus Monkeys
Toxicol Pathol, September 1, 1974; 2(3): 22 - 28.
[PDF]

Home page
 Toxicol PatholHome page
F. G. Zaki
Advanced Techniques in Biological Electron Microscopy
Toxicol Pathol, September 1, 1974; 2(3): 28 - 29.
[PDF]


HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the American Association for Clinical Chemistry.