Clinical Chemistry
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Clinical Chemistry 20: 360-364, 1974;
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Clinical Chemistry, Vol 20, 360-364, Copyright © 1974 by the American Association for Clinical Chemistry

Micromethod for Determining Total Iron-Binding Capacity by Flameless Atomic Absorption Spectrophotometry

Yu-Yan Yeh 1 and Paulus Zee 1

1 Laboratory of Nutrition and Metabolism, St. Jude Children's Research Hospital, Memphis, Tenn. 38101.

Y. Y. Yee, Laboratory of Nutrition and Metabolism, St. Jude Children's Research Hospital, P. O. Box 318, Memphis, Tenn. 38101.

Because of the large quantities of venous blood required, assays of total iron-binding capacity (TIBC) and serum iron are often omitted in diagnostic tests involving small children. As an alternative to the conventional method of assessing TIBC, we developed a micromethod based on flameless atomic absorption spectrophotometry (flameless AAS). Additionally, the standard microprocedure for determining serum iron was modified so that iron concentration could be estimated from the same iron standards used to calculate TIBC. With these procedures, only 20 µl of serum is required to prepare protein-free supernate for measurement of TIBC or serum iron with an atomic absorption spectrophotometer equipped with a graphite furnace and a recorder. The resulting TIBC, serum iron, and computed transferrin saturation values compared well with those obtained by conventional flame atomic absorption spectrophotometry. The correlation was statistically significant at the 99% confidence level. These flameless AAS micromethods provide an accurate and sensitive means of determining both TIBC and serum iron in a total of 40 µl of serum, a quantity easily collected from a fingertip puncture, and hence are appropriate for routine laboratory work in pediatric clinics and for large-scale nutrition surveys.


Key Words: pediatric chemistry • nutrition survey method • iron in serum • transferrin saturation values • comparison with conventional AAS • diagnosis of Fe-deficiency anemia • trace elements

Accepted on December 18, 1973




The following articles in journals at HighWire Press have cited this article:


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P. Zee, M. DeLeon, P. Roberson, and C.-H. Chen
Nutritional Improvement of Poor Urban Preschool Children: A 1983-1977 Comparison
JAMA, June 14, 1985; 253(22): 3269 - 3272.
[Abstract] [PDF]


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Copyright © 1974 by the American Association for Clinical Chemistry.