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Clinical Chemistry 20: 369-375, 1974;
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Clinical Chemistry, Vol 20, 369-375, Copyright © 1974 by the American Association for Clinical Chemistry

Parathyroid Hormone Assay in Primary Hyperparathyroidism: Experiences with a Radioimmunoassay Based on Commercially Available Reagents

M. Kleerekoper 1, J. P. Ingham 1, S. W. McCarthy 1, and S. Posen 1

1 Departments of Medicine and Morbid Anatomy, Sydney Hospital, Sydney, N. S. W., Australia, 2000.

A radioimmunoassay is described for parathyroid hormone in human serum, in which commercially available reagents are used almost exclusively. This assay can be done by any laboratory with experience in radloimmunoassay. Thirty-two of thirty-three patients with surgically proven primary hyperparathyroidism had detectable concentrations of parathyroid hormone in their serum, and concentrations of the hormone exceeded the normal range in 24 of them. Significant positive correlations were found between pre-operative serum calcium, pre-operative serum parathyroid hormone, and the weight of parathyroid tissue removed at operation. These three parameters were also significantly correlated with severity of the skeletal changes as assessed by semiquantitative histological methods.


Key Words: indexes to hyperparathyroidism • diagnostic aid • calculous disease • parathyroid adenoma • normal values

Submitted on October 30, 1973
Accepted on December 28, 1973




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Copyright © 1974 by the American Association for Clinical Chemistry.