Clinical Chemistry
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Clinical Chemistry 20: 376-382, 1974;
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Clinical Chemistry, Vol 20, 376-382, Copyright © 1974 by the American Association for Clinical Chemistry

Quantitation of Serum IgG by Solid-Phase Radioimmunoassay

Leo P. Cawley 1, William L. Goodwin 1, Marilyn Moeder 1, and Paulette Dibbern 1

1 K. T. Wiedemann Institute of Clinical Immunology, Wesley Medical Research Foundation; and the Department of Laboratory Medicine, Wesley Medical Center, Wichita, Kan. 67214.

Human serum immunoglobulins are most frequently measured by single gel diffusion. Radioimmunoassay by use of antibodies bonded to particles is faster and more sensitive, precise, accurate, and specific. We adopted a solid-phase radioimmunoassay (SPRIA) technique, developed in our laboratory, to measure serum IgG, and compared the results with those of a gel-diffusion method commonly used to measure serum IgG. The SPRIA technique is based on competition set up between antibodies to human IgG chemically bonded to cellulose particles and a purified serum IgG labeled with 125I. The solid phase permits prompt separation of the bound from free labeled reagent. We studied precision, accuracy, sensitivity, and specificity, as compared to gel diffusion; values for the two methods agreed well for 57 sera from hospital patients. The SPRIA procedure is more precise, and can be completed in 3 h, as compared to the 16-24 h normally required for gel diffusion procedures. The possible automation of SPRIA is discussed.


Key Words: radial gel diffusion method compared • immunologic disorders, diagnosis • cellulose-bonded IgG • interconversion of units

Submitted on October 29, 1973
Accepted on January 4, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.