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Clinical Chemistry 20: 430-435, 1974;
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Clinical Chemistry, Vol 20, 430-435, Copyright © 1974 by the American Association for Clinical Chemistry

Protein-Binding Assay for Plasma Testosterone, after Purification by Column Partition Chromatography

E. D. Horgan 1 and W. J. Riley 1

1 Department of Biochemistry, Royal Perth Hospital, Perth, Western Australia.

We describe a relatively simple, specific, and practicable method for measuring plasma testosterone by competitive protein-binding analysis. An ether extract is purified by a single chromatographic step on a micro-scale Celite—ethylene glycol column. Serum from women in the third trimester of pregnancy is used as the binding protein, and an ammonium sulfate precipitation step is used to separate the free and protein-bound testosterone. The method has a consistently low blank [0.59 ± 0.26 (SD) pmol/sample] and shows good precision. The mean testosterone concentration in normal men and menstruating women was 17.1 ± 4.0 (SD) and 1.2 ± 0.4 (SD) nmol/liter, respectively.


Key Words: normal values • competitive protein-binding analysis • Celite-ethylene glycol

Submitted on October 11, 1973
Accepted on January 14, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.