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Clinical Chemistry 20: 476-481, 1974;
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Clinical Chemistry, Vol 20, 476-481, Copyright © 1974 by the American Association for Clinical Chemistry

Clinical Evaluation of Kinetic Enzymatic Fixed-Time and Integral Analysis of Serum Triglycerides

T. O. Tiffany 1, J. M. Morton 1, E. M. Hall 1, and A. S. Garrett Jr. 1

1 Molecular Anatomy Program1 and Health Division, Oak Ridge National Laboratory, Oak Ridge, Tenn. 37830.

Serum triglycerides can be enzymatically determined by hydrolysis of triglycerides to glycerol and free fatty acids [Clin. Chem. 19, 476 (1973)]. Enzyme integral (end-point) and fixed-time rate analyses of serum triglycerides by this procedure have been investigated with a centrifugal analyzer. We determined the precision of blank determinations, the contribution of normal serum alkaline phosphatase to total values, and the amount of endogenous glycerol in fasting serum samples and blood collection tubes. We also made a statistical analysis of enzymatic triglyceride values obtained for a healthy, fasting (12-16 h) population of men and women. The relative merits of both procedures are discussed.


Key Words: centrifugal analyzer • "kit" methods • normal values • sex- and age-related effects • kinetic enzyme assay • blood glucose

Submitted on November 19, 1973
Accepted on January 31, 1974




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