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Clinical Chemistry 20: 606-607, 1974;
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Clinical Chemistry, Vol 20, 606-607, Copyright © 1974 by the American Association for Clinical Chemistry

Enzymatic Blood Glucose Determination by Colorimetry of N,N-Diethylaniline-4-Aminoantipyrine

Peter Kabasakalian 1, Sami Kalliney 1, and Anita Westcott 1

1 Research Division, Schering Corp., Bloomfield, N.J. 07003.

The hydrogen peroxide produced from glucose by glucose oxidase action at pH 7 is determined by the peroxidase oxidative coupling of N,N-diethylaniline with 4-amino-antipyrine. A 20-µl sample of plasma or serum is reacted at room temperature for 10 min. The sensitivity of the method is such that 60 µg of glucose (300 mg/dl) in a final volume of 5 ml gives an absorbance of 0.8 at 553 nm with a 1-cm cell. Absorbances are linearly related to glucose concentrations as high as 10.00 g/liter.

Submitted on November 21, 1973
Accepted on February 15, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.