Clinical Chemistry
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Clinical Chemistry 20: 649-655, 1974;
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Clinical Chemistry, Vol 20, 649-655, Copyright © 1974 by the American Association for Clinical Chemistry

Comparison of Five Methods for Determination of 2,3-Diphosphoglycerate in Blood

A. J. Teunissen 1, R. J. M. de Leeuw 1, A. B. T. J. Boink 1, M. L. Hamelink 1, and A. H. J. Maas 1

1 Departments of Cardiology, Cardiovascular Surgery and Lung Diseases, University Hospital, Utrecht, The Netherlands.

Because the importance of 2,3-diphosphoglycerate in the regulation of the oxygen affinity of hemoglobin is recognized, there is growing need for a suitable method for determining this metabolite. Here we compare the colorimetric method of de Leeuw, two enzymatic rate-dependent methods (of Nygaard and Rörth and of Loos and Prins), and two enzymatic end-point methods (of Keitt and of Ericson and de Verdier). A good linearity was observed for all methods, up to 5.0 mmol of 2,3-diphosphoglycerate per liter of blood, except for the method of Ericson and de Verdier, for which an inexplicable upward drift in the calibration curve was found. Within-run precision in the duplicates and long-term precision in the methods of Loos and Prins and of de Leeuw were excellent. Mean analytical recoveries for all the methods ranged from 97 to 100%. In a paired comparison study of the methods, with the method of Loos and Prins as a reference, we did not find statistically significant differences. Coefficients of correlation were better than 0.990 (P < 0.0005).


Key Words: " kit" methods • blood preservation • hypoxic conditions • colorimetry • enzymatic methods • hematology

Submitted on February 7, 1974
Accepted on March 26, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.