Clinical Chemistry
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Clinical Chemistry 20: 660-665, 1974;
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Clinical Chemistry, Vol 20, 660-665, Copyright © 1974 by the American Association for Clinical Chemistry

Quality-Control System for Blood pH and Gas Measurements, with Use of a Tonometered Bicarbonate—Chloride Solution and Duplicate Samples of Whole Blood

Daniel C. Noonan 1 and Robert W. Burnett 1

1 Clinical Chemistry Laboratory, Hartford Hospital, Hartford, Conn. 06115.

We describe a procedure for the simultaneous quality control of pH, pco2,and po2 measurement. A solution of NaCl (150 mmol/liter) and NaHCO3 (50 mmol/liter) is equilibrated at room temperature (24 ± 2 °C) with a gas having the composition 21% O2, 12% CO2, 67% N2. When this solution is analyzed at 37 °C in a conventional blood-gas analyzer, a pH of 7.20, a pco2 of 110 mmHg (14.6 kPa) and a po2 of 170 mmHg (22.6 kPa) are obtained. We show that data from such an analysis, performed daily, can be simply used to meet many of the unique demands of quality control in blood pH and gas determinations. This same approach may also become the basis for devising an ideal aqueous system for blood gas calibration.


Key Words: carbon dioxide electrode • oxygen electrode • po2 • pco2

Submitted on January 23, 1974
Accepted on March 31, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.