Clinical Chemistry
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Clinical Chemistry 20: 676-681, 1974;
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Clinical Chemistry, Vol 20, 676-681, Copyright © 1974 by the American Association for Clinical Chemistry

Immunochemical Measurement of Lipoprotein-X

Gerhard M. Kostner 1, Walter Petek 1, and Anton Holasek 1

1 Institute of Medical Biochemistry, University of Graz, A-8010 Graz, Austria.

Lipoprotein-X, an abnormal lipoprotein that is specific for cholestasis, was quantitated by immunochemical methods. Interfering lipoproteins also present in normal serum and sharing antigenic determinants with lipoprotein-X were removed before the sample was applied, by precipitation with purified anti-lipoprotein B or a ggr-globulin fraction of specific lipoprotein B antiserum. On Laurell electrophoresis, peak height was linearly related to lipoprotein-X concentration in the range 0.20-10 g/liter of serum. Sensitivity could be increased further by staining the plates. The coefficient of variation was less than 5%. Single radial immunodiffusion (Mancini et al. technique) was somewhat less sensitive and accurate. Results were available after 3 h by Laurell's electroimmunodiffusion technique, and after 72 h by the technique of Mancini et al. Equivalent results were obtained for samples of lipoprotein-X of extrahepatic or intrahepatic origin.


Key Words: "rocket" electroimmunodiffusion • diagnostic aid • radial immunodiffusion • hepatic disease • cholestasis • liver-function test

Submitted on March 15, 1974
Accepted on April 11, 1974




The following articles in journals at HighWire Press have cited this article:


Home page
 JPEN J Parenter Enteral NutrHome page
D. Rigaud, P. Serog, A. Legrand, M. Cerf, M. Apfelbaum, and S. Bonfils
Quantification of Lipoprotein X and its Relationship to Plasma Lipid Profile during Different Types of Parenteral Nutrition
JPEN J Parenter Enteral Nutr, September 1, 1984; 8(5): 529 - 534.
[Abstract] [PDF]


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Copyright © 1974 by the American Association for Clinical Chemistry.