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Clinical Chemistry 20: 687-691, 1974;
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Clinical Chemistry, Vol 20, 687-691, Copyright © 1974 by the American Association for Clinical Chemistry

Contamination of Distilled Water, HCl, and NH4OH with Amino Acids, Proteins, and Bacteria

Paul B. Hamilton 1 and T. Timothy Myoda 1

1 Departments of Biochemistry and Microbiology, Alfred I. du Pont Institute, Box 269, Wilmington, Del. 19899.

Contamination of distilled water with free and bound amino acids and with microbes has been overlooked in most laboratories. Unless special precautions are taken, distilled water will be contaminated with air borne microbes, probably psuedomonads, which multiply in water of conductivity quality. Amino acids and proteins that may be found in the water therefore presumably are derived from these airborne organisms. Chemical reagents such as hydrochloric acid and ammonium hydroxide solutions, which have water as an integral part, also have been found to be contaminated with amino acids. This paper deals with the extent of such contamination and describes means to provide water and hydrochloric acid (6 mol/liter) that would be suitable for determination of amino acids in concentrations of 10-9 mol/liter or less in materials of biological interest as well as rocks, lunar soil, meteorites, etc.


Key Words: analysis on the ultramicro scale • purity of reagents • preparation of uncontaminated water

Submitted on February 18, 1974
Accepted on April 11, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.