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Clinical Chemistry 20: 695-697, 1974;
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Clinical Chemistry, Vol 20, 695-697, Copyright © 1974 by the American Association for Clinical Chemistry

Use of Co-Current Dialysis in an Improved Estimation of Free Thyroxine in Serum

Herbert S. Strickler 1, Susan S. Link 1, and Robert C. Grauer 1

1 Section of Research in Endocrinology and Metabolism, William H. Singer Memorial Research Institute, Department of Laboratory Medicine, Allegheny General Hospital, 320 E. North Ave., Pittsburgh, Pa. 15212.

A new method is described for measuring free thyroxine in serum, in which co-current dialysis is used to simplify and speed the determination. The patient's serum is mixed with 125I-labeled thyroxine and buffer (28 ml of serum per deciliter) and co-currently dialyzed for 15 min against a mixture of pooled serum (8 ml/dl) in buffer. After dialysis, the free thyroxine of the patient's serum, which is now bound to the protein of the recipient stream (pooled serum), is precipitated with trichloroacetic acid. The radioactivity of this precipitate and of an undialyzed serum aliquot is measured, to obtain the total count. Use of pooled serum and trichloroacetic acid eliminates the need for the amounts of carrier thyroxine required in most other methods. The procedure is both simple and rapid (3 to 4 samples/unit/h). The values for free thyroxine correlate well with those obtained by the method of Lee and Pileggi [Clin. Chem. 17, 166 (1971)] and by others participating in the proficiency program of the College of American Pathologists.


Key Words: AutoAnalyzer • continuousflow dialysis

Submitted on February 18, 1974
Accepted on April 12, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.