Clinical Chemistry
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Clinical Chemistry 20: 765-768, 1974;
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Clinical Chemistry, Vol 20, 765-768, Copyright © 1974 by the American Association for Clinical Chemistry

Fluorometric Analysis for N'-Formylkynurenine in Plasma and Urine

T. Buxton 1 and G. G. Guilbault 1

1 Department of Chemistry, Louisiana State University in New Orleans, New Orleans, La. 70122.

We describe a fluorometric method of analysis for N'-formylkynurenine. The method depends on the rate of enol formation when a solution of the compound is made basic. The enol is more fluorescent than the neutral compound. The increase in fluorescence is measured, with use of 430-nm emission and 319-nm excitation wavelengths. This method is applied to the determination of N'-formylkynurenine in protein-free filtrates of plasma or urine. To our knowledge, this is the first quantitative method for determination of N'-formylkynurenine.

Submitted on February 19, 1973
Accepted on April 30, 1974




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Copyright © 1974 by the American Association for Clinical Chemistry.