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Clinical Chemistry 20: 769-774, 1974;
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Clinical Chemistry, Vol 20, 769-774, Copyright © 1974 by the American Association for Clinical Chemistry

Stability in Urea as a Measure of Cardiac Lactate Dehydrogenase on the Centrifugal Analyzer

Naomi Quast Hanson 1 and Esther F. Freier 1

1 Department of Laboratory Medicine and Pathology, University of Minnesota Medical School, Box 198, Mayo Memorial Building, Minneapolis, Minn. 55455.

Kinetic methods are described for determination of total, heat-stable, and urea-stable lactate dehydrogenase (EC 1.1.1.27) activity on the centrifugal analyzer. In the urea-stable method we present, 2.6 molar urea is used to differentiate lactate dehydrogenase originating from heart and liver. This urea concentration, greater than that used by most other investigators, better differentiates between cardiac and liver lactate dehydrogenase and is as sensitive and specific for cardiac lactate dehydrogenase as the heatstable method; excellent correlation was obtained on 200 specimens assayed by both methods. The ureastable method has the advantages of speed and simplicity, better precision, and decreased serum volume, and is, therefore, preferred to the heat-stable method for determination of cardiac lactate dehydrogenase isoenzymes.


Key Words: kinetic enzyme assay • diagnostic aid • normal values • inter-method comparison

Submitted on March 13, 1974
Accepted on May 2, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.