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Clinical Chemistry 20: 812-815, 1974;
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Clinical Chemistry, Vol 20, 812-815, Copyright © 1974 by the American Association for Clinical Chemistry

New Assay for Tryptophan and Its 5-Hydroxyindole Metabolites in Blood

George G. Guilbault 1 and Peter M. Froehlich 1

1 Department of Chemistry, Dalhousie University, Halifax, N. S. B3M 1G8, Canada.

We describe a new cation-exchange fluorometry procedure for separation and measurement of three 5-hydroxyindole metabolites of tryptophan in blood. The metabolites are detected and measured via the fluorescence emitted in the visible region when the separated metabolite is added to a hydrochloric acid-ascorbic acid mixture. The assay requires only 30 min and is both selective and sensitive. The normal concentration of 5-hydroxytryptamine (serotonin) in serum is 98 µg/liter; 5-hydroxyindole acetic acid and 5-hydroxytryptophan are normally present in concentrations of less than 20 µg/liter.


Key Words: normal values • 5-hydroxytryptamine (serotonin) • 5-hydroxyindole acetic acid • monitoring the dialyzed patient • cation-exchange chromatography • fluorometry

Submitted on March 11, 1974
Accepted on May 9, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.