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Clinical Chemistry 20: 816-819, 1974;
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Clinical Chemistry, Vol 20, 816-819, Copyright © 1974 by the American Association for Clinical Chemistry

Use of a "Three-Isoenzyme Diagram" in Assessing Activity of Alkaline Phosphatase Isoenzymes

Jae C. Kim 1, Milos Stastny 2, and John P. Manning 3

1 Technicon Instruments Corp., Tarrytown, N. Y. 10591.
2 Technicon Instruments Corp., Tarrytown, N. Y. 10591.Corning Glass Works, Biomedical Technical Center, Raleigh, N. C. 27602.
3 Saint Barnabas Medical Center, Livingston, N. J. 07039.

A "three-isoenzyme diagram" has been devised to visualize the alkaline phosphatase profile quickly. Activities of the individual isoenzymes can then be estimated from the diagram. Each vertex represents a pure alkaline phosphatase isoenzyme, thus forming a triangle with three alkaline phosphatase isoenzymes, each side of the triangle describing the concentration ratio of a binary mixture of the respective isoenzymes. The validity of the three-isoenzyme diagram was tested by mixing three sets of two abnormal sera in three proportions. Results show that linearity and proportionality agree with what would be assessed from the diagram.

Submitted on August 24, 1973
Accepted on May 9, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.