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Clinical Chemistry 20: 1023-1027, 1974;
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Clinical Chemistry, Vol 20, 1023-1027, Copyright © 1974 by the American Association for Clinical Chemistry

Instrumental Detection System Based on Light Scattering for Performing Assays in Which Agglutination Reactions Are Used

Philip Blume 1, Margaret Hanson 1, J. Mathys 1, and Herbert F. Polesky 1

1 Department of Laboratory Medicine and Pathology, University of Minnesota; and War Memorial Blood Bank, Minneapolis, Minn. 55455.

The presence of even a small extent of agglutination among erythrocytes in aqueous suspension can be ascertained by a discrete-particle light-scattering technique. This provides a means of mechanizing a wide variety of assays done in connection with blood-banking, as well as other procedures that rely upon the agglutination of sensitized particles or cells. We describe an agglutination-detection system designed to serve this function, and demonstrate its sensitivity.


Key Words: blood typing • hematology • particle counting

Submitted on May 2, 1974
Accepted on June 5, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.