Clinical Chemistry
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Clinical Chemistry 20: 1080-1085, 1974;
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Clinical Chemistry, Vol 20, 1080-1085, Copyright © 1974 by the American Association for Clinical Chemistry

DNA Cytophotometry of Chromosomes in a Case of Chronic Myelogenous Leukemia

Brian H. Mayall 1, Anthony V. Carrano 1, and Janet D. Rowley 1

1 Biomedical Division, Lawrence Livermore Laboratory, University of California, Livermore, Calif. 94550.

The DNA-stain content of human metaphase chromosomes is measured by use of quantitative cytochemistry, scanning cytophotometry, and image-processing techniques. This approach is applied to chromosomes from a female with chronic myelogenous leukemia. Measurements are normalized for each cell and expressed on a scale by which the sum of the measurements for the 44 autosomal chromosomes is 100 units; the normalized measurements are compared with standard values from eight normal persons. Banding analysis of the leukemic cells shows a 9q+/22qtranslocation. This is confirmed by the DNA measurements; 0.42 units are involved. In addition, measurements of both leukemic and nonleukemic cells reveal a 9+/20-translocation that cannot be detected visually; 0.12 units are involved. Neither translocation shows a net loss of DNA; any loss exceeding 0.05 units (6 x 106 base pairs of DNA) would be significant (P < 0.05). These results illustrate the power of this approach and bear on the etiology of chronic myelogenous leukemia.


Key Words: chromosomal DNA content, • quantitative cytochemistry • scanning cytophotometry • chromosomal translocation • Philadelphia chromosome

Submitted on May 20, 1974
Accepted on June 6, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.