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Clinical Chemistry 20: 992-997, 1974;
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Clinical Chemistry, Vol 20, 992-997, Copyright © 1974 by the American Association for Clinical Chemistry

Electrochemical Detection of Selected Organic Components in the Eluate from High-Performance Liquid-Chromatography

Peter T. Kissinger 1, Lawrence J. Felice 1, Ralph M. Riggin 1, Lawrence A. Pachla 1, and David C. Wenke 2

1 Department of Chemistry, Michigan State University, East Lansing, Mich. 48824.
2 Erie County Laboratory, 462 Grider St., Buffalo, N. Y. 14215.

High-performance liquid chromatography can be combined with hydrodynamic thin-layer electrochemistry for determination of trace amounts of organic constituents in complex samples. With small and inexpensive analyzers based on these two techniques, as little as 1 pg of an electrochemically active component can be detected in a few minutes. Because many of the important low-molecular-weight organic constituents of body fluids— both endogenous metabolites and drugs—undergo electrochemical reactions, it seems reasonable to presume that useful assays might be developed by using the above methodology. Beginning to explore this presumption, we illustrate how uric acid, ascorbic acid, catecholamines, and related tyrosine metabolites might be measured in urine and serum. In some cases, liquid chromatography with electrochemical detection provides better sensitivity, selectivity, and speed than traditional methods, while minimizing the need for analytical reagents. We describe the basic approach and progress to date and suggest future applications.


Key Words: ultramicroscale analysis • thinlayer electrochemistry • uric and ascorbic acid determination • dopa • catecholamines

Submitted on March 21, 1974
Accepted on May 25, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.