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Clinical Chemistry 20: 1105-1110, 1974;
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Clinical Chemistry, Vol 20, 1105-1110, Copyright © 1974 by the American Association for Clinical Chemistry

High-Resolution Gas-Chromatographic Analysis of the Volatile Constituents of Body Fluids, with Use of Glass Capillary Columns

Milos Novotny 1, Michael L. McConnell 1, Milton L. Lee 1, and Raleigh Farlow 1

1 Department of Chemistry, Indiana University, Bloomington, Ind. 47401.

Trace volatile constituents of human urine, serum, and cerebrospinal fluid were concentrated on a porous polymer precolumn, and resolved with high-efficiency glass capillary columns. Thin-film glass columns facilitate effective resolution of underivatized compounds within the volatility range up to docosane in relatively short analysis times. Glass capillary columns coated with stationary phases of different polarity were used. Recorded volatile metabolic profiles of human urine are altered after the sample is treated with beta-glucuronidase and sulphatase.


Key Words: serum, urine, cerebrospinal fluid • metabolic profiles • diagnostic aid • elution temperatures of reference compounds

Submitted on April 4, 1974
Accepted on May 13, 1974




The following articles in journals at HighWire Press have cited this article:


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J. W. JORGENSON, M. NOVOTNY, M. CARMACK, G. B. COPLAND, S. R. WILSON, S. KATONA, and W. K. WHITTEN
Chemical Scent Constituents in the Urine of the Red Fox (Vulpes vulpes L.) During the Winter Season
Science, February 17, 1978; 199(4330): 796 - 798.
[Abstract] [PDF]


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Copyright © 1974 by the American Association for Clinical Chemistry.