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Clinical Chemistry 20: 1111-1115, 1974;
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Clinical Chemistry, Vol 20, 1111-1115, Copyright © 1974 by the American Association for Clinical Chemistry

Differentiation of Electrophoretically Similar Hemoglobins—Such as S, D, G, and P; or A2, C, E, and O—by Electrophoresis of the Globin Chains

Rose G. Schneider 1

1 Department of Pediatrics, University of Texas Medical Branch, Galveston, Tex. 77550.

Electrophoresis of the globin chains of hemoglobin on cellulose acetate in both acidic and alkaline buffers (pH about 6 and 9) containing urea and 2-mercaptoethanol is a simple, rapid means of characterizing hemoglobins. Erythrocyte hemolysate is electrophoresed in the presence of a large amount of mercaptoethanol, which liberates heme from globin, and keeps it in solution during its rapid electrophoretic removal. Each globin chain migrates at a characteristic rate, which varies with the pH and composition of the buffer. The combined data permit differentiation, with a high degree of specificity, of some similarly charged hemoglobins. They may also be useful in assessing the effect of secondary and tertiary structure on molecular charge.


Key Words: diagnostic aid • genetic counseling

Submitted on January 25, 1974
Accepted on May 16, 1974




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Copyright © 1974 by the American Association for Clinical Chemistry.