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Clinical Chemistry 20: 1116-1120, 1974;
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Clinical Chemistry, Vol 20, 1116-1120, Copyright © 1974 by the American Association for Clinical Chemistry

Simultaneous Determination of Methemoglobin and Total Hemoglobin by a Continuous-flow Method

William A. Groff Sr. 1, Andris Kaminskis 1, and Samuel A. Cucinell 1

1 Department of the Army, Edgewood Arsenal, Biomedical Laboratory, Aberdeen Proving Ground, Md. 21010.

We describe a sensitive, accurate automated method for the simultaneous measurement of methemoglobin and total hemoglobin in blood. The method can be used for either continuous in vivo monitoring or intermittent sampling at a rate of 20/h. Standardization procedures are described, in which human blood treated with sodium nitrite is used. Repeatability of methemoglobin and total hemoglobin measurements, expressed as coefficient of variation in percent, are within ±4.1 and ±2.6%, respectively. Normal methemoglobin concentration’s in humans and dogs did not exceed 0.55 g/dl (3.3% of the total hemoglobin).


Key Words: normal values • dogs • methemoglobin production • AutoAnalyzer • in vivo monitoring • cyanide poisoning

Submitted on October 15, 1973
Accepted on May 21, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.