Clinical Chemistry
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Clinical Chemistry 20: 1125-1127, 1974;
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Clinical Chemistry, Vol 20, 1125-1127, Copyright © 1974 by the American Association for Clinical Chemistry

Automated Procedure for Determination of Sialic Acids and 2-Deoxyribose in Blood and Tissues

R. L. Engen 1, A. Anderson 1, and L. L. Rouze 1

1 Department of Veterinary Anatomy, Pharmacology, and Physiology, College of Veterinary Medicine, Iowa State University, Ames, Iowa 50010.

A manual procedure for measuring sialic acids in blood and tissue and an automated procedure for measuring sialic acids in blood have been combined and modified so that sialic acid concentrations in both blood and tissue can be determined by continuous-flow analysis. Because 2-deoxyribose interferes with tissue sialic acid determinations, there must be a correction for color produced by it. In the calculation of sialic acids, the value for 2-deoxyribose can simultaneously be obtained. In addition to the analyses of mixed standards of sialic acids and 2-deoxyribose, we report analytical data for rat blood and tissue samples.


Key Words: AutoAnalyzer • continuous-flow analysis • rats

Submitted on October 8, 1973
Accepted on May 28, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.