Clinical Chemistry
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Clinical Chemistry 20: 1128-1130, 1974;
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Clinical Chemistry, Vol 20, 1128-1130, Copyright © 1974 by the American Association for Clinical Chemistry

Validity of a Simple Automated Method in Which the "Direct" Kober Reaction is Used to Measure Estrogens during Pregnancy

Adel A. A. Ismail 1 and Susan M. Watson 1

1 Division of Clinical Chemistry, Clinical Research Centre, and Northwick Park Hospital, Harrow, Middlesex, England.

Using a simple automated procedure for measurement of total estrogens in urine, we found that several estrogen conjugates gave higher fluorescence yields than did the corresponding free steroids. In the analysis of urine samples from pregnant women, the "direct Kober reaction" gave consistently higher values than those obtained after preliminary acid or enzymatic hydrolysis. The effects of storage and urine dilution on results for estrogens were also investigated.


Key Words: Kober reaction • fluorometry • conjugated steroids • urinary estrogens

Submitted on April 1, 1974
Accepted on May 28, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.