Clinical Chemistry
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Clinical Chemistry 20: 1146-1149, 1974;
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Clinical Chemistry, Vol 20, 1146-1149, Copyright © 1974 by the American Association for Clinical Chemistry

Separation of Free and Protein-Bound Ligand Molecules by Means of Protein-Coated Charcoal

Esper Mortensen 1

1 Department of Clinical Chemistry, Kommunehospitalet, 8000 Åarhus C, Denmark.

Separation of free and protein-bound ligand molecules by means of protein-coated charcoal has been studied in model experiments. The results can be described by applying the simple laws of mass action and dilution, and indicate the need for a careful evaluation of the charcoal that is being used and a testing against an ideal separation method whenever experimental conditions are determined or changed.


Key Words: "instant dialysis" • binding characteristics of charcoal • competitive binding of ligands

Submitted on November 7, 1973
Accepted on June 9, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.