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Clinical Chemistry, Vol 20, 1150-1154, Copyright © 1974 by the American Association for Clinical Chemistry
1 Endocrinology Section, Department of Medicine, The University of Chicago, Chicago, Ill. 60637.
Simple techniques for controlling interference from binding proteins in serum, such as thyroxine-binding globulin, in radioimmunoassay for triiodothyronine (T3) have been evaluated for their efficacy, and their effect on assay sensitivity and on recovery of added T3. Ethanol precipitation of serum proteins decreased the assay sensitivity, nonspecific binding was increased, and recoveries of added T3 were inconsistent. Heat-inactivation of thyroxine-binding globulin or use of 8-anilino-1naphthalene sulfonic acid (ANS) to displace T3 from thyroxine-binding globulin produced comparable recovery rates. The heat-inactivation method slightly decreased the sensitivity of the assay and prolonged the procedure, whereas use of ANS is simple, and the assay sensitivity is maintained. When sera contain a high concentration of thyroxine-binding globulin, a fixed concentration of ANS (175 µg/100 µl of serum) might be too low to displace T3 from all its binding sites, but a concentration of ANS greater than 200 µg/100 µl of serum interferes with T3 quantitation by competitively binding to the antibodies. The cross-reactivity of thyroxine to T3-antibodies varies with the antiserum. Thyroxine-binding globulin appears to be the only protein in serum that competes with the antibody for T3 binding.
Submitted on April 3, 1974
Accepted on June 11, 1974
The following articles in journals at HighWire Press have cited this article:
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 T. Sunthornthepvarakul, S. Likitmaskul, S. Ngowngarmratana, K. Angsusingha, S. Kitvitayasak, N. H. Scherberg, and S. Refetoff Familial Dysalbuminemic Hypertriiodothyroninemia: A New, Dominantly Inherited Albumin Defect J. Clin. Endocrinol. Metab., May 1, 1998; 83(5): 1448 - 1454. [Abstract] [Full Text]
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R. E. Van Pelt, P. P. Jones, K. P. Davy, C. A. DeSouza, H. Tanaka, B. M. Davy, and D. R. Seals Regular Exercise and the Age-Related Decline in Resting Metabolic Rate in Women J. Clin. Endocrinol. Metab., October 1, 1997; 82(10): 3208 - 3212. [Abstract] [Full Text] [PDF]
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 G. D. Lumb and J. H. Rust The Pathologic Response of the Liver and Thyroid of the Rat to Potassium Prorenoate (SC-23992) Toxicol Pathol, June 1, 1985; 13(4): 315 - 324. [Abstract] [PDF]
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 M. E. BROWN and S. REFETOFF Transient Elevation of Serum Thyroid Hormone Concentration After Initiation of Replacement Therapy in Myxedema Ann Intern Med, April 1, 1980; 92(4): 491 - 495. [Abstract] [PDF]
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