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Clinical Chemistry 20: 1173-1180, 1974;
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Clinical Chemistry, Vol 20, 1173-1180, Copyright © 1974 by the American Association for Clinical Chemistry

Determination of Short-Chain Fatty Acids in Stool Ultrafiltrate and Urine

Dennis P. Collin 1 and Patrick G. McCormick 1

1 Department of Chemistry, Marquette University, Milwaukee, Wis. 53233.

A new method is reported for determining total shortchain fatty acids in stool water and urine. The method uses ion-exchange resins to remove interfering substances, and determines these acids by direct titration. Lactic acid, if present, is determined along with the short-chain (C1-C6) organic acids. Interferences such as carbonate, phosphate, creatinine, bile acids, and uric acid are removed by the ion-exchange treatment. Values obtained by this simplified technique correlate very well with those by gas-chromatographic analyses of the same sample, and the method represents a significant improvement over the Van Slyke technique.


Key Words: ion-exchange chromatography • volatile fatty acids • intestinal function assessment • gas chromatography

Submitted on March 7, 1974
Accepted on June 18, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.