Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 20: 1187-1193, 1974;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by Helman, E. Z.
Right arrow Articles by Holland, S.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by Helman, E. Z.
Right arrow Articles by Holland, S.

Clinical Chemistry, Vol 20, 1187-1193, Copyright © 1974 by the American Association for Clinical Chemistry

Elimination of Error Caused by Hemolysis and Bilirubin-induced Color Quenching in Clinical Radioimmunoassays

Edith Zak Helman 1, Vina Spiehler 1, and Susan Holland 1

1 Radioimmunoassay Center, SID Applications Research, Beckman Instruments, Inc., Campus Drive at Jamboree Blvd., Irvine, Calif. 92664.

In radioimmunoassays and radioassays for various constituents of serum, color quenching is a source of error when liquid-scintillation methods are used in samples that contain hemolyzed blood and samples from jaundiced patients. A quench correction can be made by using the External Standard Channels Ratio method and normalizing the ratios, or, alternatively, by chemical decolorization with tetramethylammonium hydroxide, hydrogen peroxide, and ascorbic acid.

Submitted on April 15, 1974
Accepted on June 4, 1974






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1974 by the American Association for Clinical Chemistry.