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Clinical Chemistry 20: 1231-1233, 1974;
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Clinical Chemistry, Vol 20, 1231-1233, Copyright © 1974 by the American Association for Clinical Chemistry

Automated Enzymatic Micromethod for Determination of Uric Acid in Serum and Urine with a Centrifugal Analyzer

Michael A. Pesce 1, Selma H. Bodourian 1, and John F. Nicholson 1

1 Division of Clinical Chemistry, Babies Hospital, The Children’s Medical and Surgical Center of New York at Columbia-Presbyterian Medical Center; and The Department of Pediatrics, College of Physicians and Surgeons of Columbia University, 3975 Broadway, New York, N. Y. 10032.

Uric acid is determined in serum and urine by an enzymatic method on a centrifugal analyzer. The sample (8 µl) is mixed with the enzyme uricase in a sodium borate buffer. The resulting decrease in absorbance at 292 nm is linearly proportional to the concentration of uric acid from 1 to 75 mg of uric acid per deciliter. Use of a centrifugal analyzer eliminates measurement of a separate serum blank. Recovery of uric acid added to serum and urine averaged 100% (range: 97-110%). Within-run precision (CV) on a serum pool for which the mean value was 5.96 mg/dl was 2.52%; day-to-day precision, measured on the same serum pool over a seven-day period, was 2.81% (mean: 6.05 mg/dl). Results obtained for serum with the uricase method on a centrifugal analyzer and with the uricase method on the Du Pont aca yielded a correlation coefficient of 0.99.


Key Words: Du Pont aca • CentrifiChem • uricase

Submitted on May 10, 1974
Accepted on June 13, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.