Clinical Chemistry
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Clinical Chemistry 20: 1235-1237, 1974;
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Clinical Chemistry, Vol 20, 1235-1237, Copyright © 1974 by the American Association for Clinical Chemistry

Quantitative Gas-Chromatographic Determination of Short-Chain Fatty Acids in Aqueous Samples

Dennis P. Collin 1, Patrick G. McCormick 2, and Milton G. Schmitt Jr. 3

1 Department of Chemistry, Marquette University, Milwaukee, Wis. 53233.Department of Gastroenterology, Walter Reed Army Institute of Research, Washington, D. C. 20012.
2 Department of Chemistry, Marquette University, Milwaukee, Wis. 53233.Pfizer, Inc., Quality Control Division, Brooklyn, N. Y. 11206.
3 Department of Gastroenterology, Medical College of Wisconsin, Milwaukee, Wis. 53226. Present address: Rockford Clinic, 2300 North Rockton Ave., Rockford, Ill. 61101.

We report the use of SP-1200 (Supelco Inc.) for quantitative gas-chromatographic determination of short-chain fatty acids (C2-C5) in stool water. The ratio of peak areas for these acids to that for 2-methylvaleric acid (internal standard) is linear for each acid from 60 to 1800 mg/liter. Lactic acid, occasionally present in stool in abnormally high amounts, is not detectable as the free acid on SP-1200, but is determined as its propyl ester on diethyleneglycolsuccinate.


Key Words: diagnostic aid • gas chromatography of aqueous samples • analysis of stool water lipids

Submitted on June 3, 1974
Accepted on July 11, 1974






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Copyright © 1974 by the American Association for Clinical Chemistry.