Clinical Chemistry
HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
QUICK SEARCH:   [advanced]


     

Clinical Chemistry 21: 109-112, 1975;
This Article
Right arrow Full Text (PDF)
Right arrow Alert me when this article is cited
Right arrow Alert me if a correction is posted
Services
Right arrow Email this article to a friend
Right arrow Similar articles in this journal
Right arrow Similar articles in PubMed
Right arrow Alert me to new issues of the journal
Right arrow Download to citation manager
Right arrow reprints & permissions
Citing Articles
Right arrow Citing Articles via Google Scholar
Google Scholar
Right arrow Articles by McIntyre, J. A.
Right arrow Articles by Haberfeldel, G. C.
Right arrow Search for Related Content
PubMed
Right arrow PubMed Citation
Right arrow Articles by McIntyre, J. A.
Right arrow Articles by Haberfeldel, G. C.

Clinical Chemistry, Vol 21, 109-112, Copyright © 1975 by the American Association for Clinical Chemistry

Thin-layer Chromatography and Enzyme Immunoassay of L-Alpha-Acetyl Methadol and Methadone Metabolites in Urine

Joyce A. McIntyre 1, Arlene E. Armandi 1, Lance P. Risen 1, Walter Ling 1, and George C. Haberfeldel 1

1 Department of Pathology, Veterans Administration Hospital, Sepulveda, Calif. 91343 and the Department of Psychiatry, University of California at Los Angeles, Los Angeles, Calif. 90024.

We describe a thin-layer chromatographic technique suitable for isolating at least four metabolites of L-agr-acetyl methadol. The metabolites are discussed in terms of their color reactions to chromatography spray reagents and their appearance in the urine during various time intervals after dosage. Methadone is detected by the presence of the unmetabolized drug and two of its metabolites in the urine. We present a simple means of distinguishing methadone-containing specimens from those that contain L-agr-acetyl methadol. Results of an immunoassay for methadone applied to specimens from patients receiving L-agr-acetyl methadol or methadone are discussed. The method is suitable as a routine screening procedure for most laboratories that use thin-layer chromatography.


Key Words: toxicology • screening • drug abuse

Submitted on May 20, 1974
Accepted on October 18, 1974






HOME HELP FEEDBACK SUBSCRIPTIONS ARCHIVE SEARCH TABLE OF CONTENTS
Copyright © 1975 by the American Association for Clinical Chemistry.