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Clinical Chemistry 21: 125-129, 1975;
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Clinical Chemistry, Vol 21, 125-129, Copyright © 1975 by the American Association for Clinical Chemistry

Simplified Automated Method for Determination of Urinary or Serum Uric Acid, Based on Reduction of Ferric-Phenanthroline Complex

John W. Nelson 1 and K. K. Batra 1

1 Solano Laboratories, 2920 Telegraph Ave., Berkeley, Calif. 94705.

We describe an automated colorimetric method for determination of uric acid in serum or urine by use of an AutoAnalyzer II or SMA 12/60 (Technicon Corp.). The method depends on reduction of a ferric-phenanthroline complex by uric acid under acidic conditions to a ferrous-phenanthroline complex, which absorbs at 505 nm. Advantages of this method over other methods now in use are that color and concentration are linearly related (to 20 mg/100 ml); aqueous reagents are easily prepared, stable, and inexpensive; and interference from ascorbic acid has been eliminated by use of an alkaline copper-containing diluent. The analysis is also free of interference from glucose, creatinine, glutathione, salicylates and hemoglobin. Correlation with results of the Technicon phosphotungstic acid reduction method is excellent (r = .996). Correlation with results of an automated uricase method is satisfactory (r = .979). Recovery of uric acid was 101% over a wide concentration range.

Submitted on October 3, 1974
Accepted on October 21, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.