Clinical Chemistry
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Clinical Chemistry 21: 154-155, 1975;
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Clinical Chemistry, Vol 21, 154-155, Copyright © 1975 by the American Association for Clinical Chemistry

Inactivated Serum vs. Saline as a Diluent in Some Serum Enzyme Assays

Ted W. Fendley 1 and Christopher S. Frings 1

1 Medical Laboratory Associates, A Damon Laboratory, 1025 So. 18th St., Birmingham, Ala. 35205.

In assays of creatine kinase (EC 2.7.3.2), alkaline phosphatase (EC 3.1.3.1), lactate dehydrogenase (EC 1.1.1.27), or asparate aminotransferase (EC 2.6.1.1) activities by certain end-point methods, we found no significant difference in the accuracy and precision of the results when serum was diluted with heat-inactivated pooled serum or NaCl solution (8.5 g/liter). For creatine kinase, asparate aminotransferase, and alkaline phosphatase, correlations were good for the two diluents, but poor in the case of lactate dehydrogenase.

Accepted on October 19, 1974






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Copyright © 1975 by the American Association for Clinical Chemistry.