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Clinical Chemistry 21: 1416-1421, 1975;
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Clinical Chemistry, Vol 21, 1416-1421, Copyright © 1975 by the American Association for Clinical Chemistry

Use of the Reference Method for Determination of Serum Calcium in a Quality-Assurance Survey

John F. Pickup 1, Malcolm J. Jackson 2, E. Margaret Price 3, Michael J. R. Healy 4, and Stanley S. Brown 1

1 Division of Clinical Chemistry, Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ, England.
2 Department of Human Metabolism, University College Hospital, Gower Street, London WC1, England.;
3 University College Hospital Medical School, University Street, London WC1, England.
4 Division of Computing and Statistics, Clinical Research Centre, Watford Road, Harrow, Middlesex HA1 3UJ, England.

The Reference Method [Clin. Chem. 19, 1208 (1973)] for determination of total calcium in serum has been applied to 29 specimens from the Wellcome Group Quality-Control Programme. The accuracy of the mean resuIts for all participants, and for selected groups of participants, was determined over the concentration range 2.00-2.75 mmol/liter. We show that, in general, continuous-flow (AutoAnalyzer) and atomic absorption methods of analysis have a small, constant bias, but chelation (ethylenediaminetetraacetate) methods are unpredictably inaccurate.

Submitted on November 12, 1974
Accepted on May 23, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.