Clinical Chemistry
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Clinical Chemistry 21: 1437-1440, 1975;
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Clinical Chemistry, Vol 21, 1437-1440, Copyright © 1975 by the American Association for Clinical Chemistry

Quantitative Method for Estimating Fecal Amino Acids

Christopher W. I. Owens 1 and Walfredo Padovan 1

1 Medical Unit, University College Hospital Medical School, University of London, London, W.C.1., England.

We describe a method for quantitatively estimating 24 ninhydrin-reacting substances, including the commoner amino acids, in fecal dialysate prepared from ingested dialysis bags retrieved from fresh stool. It is accurate to 2 µmol of agr-amino nitrogen per liter of fecal dialysate, and for most substances recovery of added standards is 100%. It involves dilution, ultrafiltration, and automated ion-exchange column chromatography of the dialysate. Some normal values are provided.


Key Words: fecal dialysate • ultrafiltration • cation-exchange chromatography • fecal "free" amino acids • colonic metabolism • normal (reference) values • gastrointestinal physiology and pathology • stool water

Submitted on April 3, 1975
Accepted on June 2, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.