Clinical Chemistry
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Clinical Chemistry 21: 1441-1447, 1975;
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Clinical Chemistry, Vol 21, 1441-1447, Copyright © 1975 by the American Association for Clinical Chemistry

Critical Assessment of Phospholipid Measurement in Amniotic Fluid

Linda P. Badham 1 and Howard G. J. Worth 1

1 Department of Clinical Chemistry, Birmingham Maternity Hospital, Queen Elizabeth Medical Centre, Birmingham B15 2TG, U. K.

We assessed several methods of inorganic phosphate assay for their suitability in estimating phospholipids in digested extracts of amniotic fluids. The extraction and digestion procedures used for phospholipids from amniotic fluid were also examined critically. The effect of contamination by blood or obstetric cream has been examined. Accordingly, we suggest a method for measuring total phospholipids in amniotic fluids, and results of it are compared with the lecithin/sphingomyelin ratio measurement in some clinical situations.


Key Words: lecithin/sphingomyelin ratio • respiratory distress syndrome • Index of fetal viability • intermethod comparison

Submitted on March 5, 1975
Accepted on June 9, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.