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Clinical Chemistry 21: 1448-1453, 1975;
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Clinical Chemistry, Vol 21, 1448-1453, Copyright © 1975 by the American Association for Clinical Chemistry

Optimum Kinetic Enzymatic Procedures for Glucose and Triglycerides in Plasma and Serum

Joseph L. DiCesare 1

1 The Perkin-Elmer Corp., Main Ave., Norwalk, Conn. 06856.

I describe optimum kinetic procedures for measuring glucose and triglycerides in plasma and serum by enzymatic techniques. Glucose was measured by the hexokinase method at a 1000-fold sample dilution. Absorbance and concentration are linearly related for concentrations up to 4 g/liter. Results are reproducible to about 25 mg/liter (standard error). Comparisons between the kinetic glucose method described here and an automated o-toluidine and a manual end-point method showed no apparent bias between the methods; correlation coefficients were 0.998 and 0.991, respectively. Triglycerides (triacylglycerols) were measured in a fully enzymatic system by measuring free glycerol after hydrolysis with lipase. Absorbance and concentration are linearly related to >2.5 g/liter at a 300-fold sample dilution. Repeatability was about 30 mg/liter (standard error). Compared with a manual method, the correlation coefficient was 0.978, with a slope of 0.93.


Key Words: intermethod comparison • "kit" methods • normal (reference) values

Submitted on May 7, 1975
Accepted on June 13, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.