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Clinical Chemistry 21: 1454-1456, 1975;
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Clinical Chemistry, Vol 21, 1454-1456, Copyright © 1975 by the American Association for Clinical Chemistry

Modification of the Fiske and SubbaRow Method for Total Phospholipid in Serum

Herbert K. Naito 1

1 Division of Research and Division of Laboratory Medicine, The Cleveland Clinic Foundation, 9500 Euclid Ave., Cleveland, Ohio 44106.

Many of the difficulties encountered in the original Fiske and SubbaRow method for estimating serum phospholid were corrected through the use of vanadium pentoxide, a catalyst used in sample digestion, and through other minor modifications. Results obtained by the modified method agree well with those obtained by three other established phospholipid procedures. The greatly improved speed, simplicity, accuracy, and precision now makes the modified Fiske and SubbaRow method ideal for clinical or research use.

Submitted on May 2, 1975
Accepted on June 12, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.