Clinical Chemistry
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Clinical Chemistry 21: 1469-1473, 1975;
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Clinical Chemistry, Vol 21, 1469-1473, Copyright © 1975 by the American Association for Clinical Chemistry

Simplified Photometric Copper-Soap Method for Rapid Assay of Serum Lipase Activity

James F. Myrtle 1 and Wilbert J. Zell 1

1 Research Department, Bio-Science Laboratories, 7600 Tyrone Ave., Van Nuys, Calif. 91405.

We modified the method of Yang and Biggs [Clin. Chem. 17, 512 (1971)] for greater speed and simplicity. Serum is incubated with an emulsified olive oil substrate, the fatty acids extracted, the copper soap formed, and phases separated, all in a single tube. An aliquot of the organic phase is removed for color development. One analysis can be completed in 35 min, 20 in less than 2 h. The method is accurate and precise. Results correlate well with the unmodified colorimetric method, and with the classical titrimetric lipase procedure. The reference interval (normal range) is 20 to 180 U/liter.


Key Words: pancreatic disease • intermethod comparison • normal values (reference interval)

Submitted on February 26, 1975
Accepted on June 19, 1975






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Copyright © 1975 by the American Association for Clinical Chemistry.